Field data collection complete Lab analysis underway

California Biodiversity

California has committed to protecting 30% of its land by 2030, but much of what lives there has never been counted. Thousands of volunteer-collected soil and insect samples are now filling that gap in the state’s biodiversity inventory

See the results

Results

Statewide data from places researchers rarely reach

63%
of California's 177 ecoregions sampled by volunteers, 112 in all
3,100+
soil and insect samples delivered to UC Santa Cruz and the Natural History Museum of Los Angeles County
580
volunteers collecting under one standardized scientific protocol
The quality of the samples are good... We were able to reach such a broad geographic scope across California, so it really wouldn't have been possible without all of those community scientists going out and collecting soil for us.
Ajith Seresinghe, University of California, Santa Cruz

Where volunteers went

Off the trail and up the 4x4 roads

Volunteers chose from designated sample sites across the state. Many sat on Bureau of Land Management parcels and national forests, where reaching the pin meant high-clearance roads, creek crossings, or long hikes in.

At each site, volunteers logged the sample in Survey123 with photos and a barcode scan, then shipped it straight to the lab that would analyze it. Lab partners checked every sample against its field record before analysis.

  • Amargosa Desert
  • Northern Sierra Nevada
  • Santa Lucia Range
  • Venturan-Angeleno hills
  • Southern California mountains
Map of California covered in sample points from the northern mountains and Sierra Nevada to the southern coastal ranges and deserts.
Sampled site

From trail to lab

Every sample has a destination

  1. 1
    Adventure Scientists volunteers

    Collect

    Soil and insect samples from designated sites, logged on location with photos and a barcode scan.

  2. 2
    UC Santa Cruz

    Sequence

    Extracts environmental DNA from each soil sample and adds it to a statewide soil biodiversity archive.

  3. 3
    Natural History Museum of LA County

    Identify

    Sorts and identifies the insects, including rare finds that take many eyes in the field to catch.

  4. 4
    California Institute for Biodiversity

    Inventory

    Brings results into the California All Taxa Biodiversity Inventory, a baseline for conservation planning.

Why it matters

Conservation needs a baseline

California is one of the most biodiverse places on Earth, and it has committed to conserving 30% of its lands and coastal waters by 2030. Choosing which places matter most starts with knowing what lives where.

For insects and soil life, the fungi, nematodes, and bacteria that hold ecosystems together, much of that baseline is missing. Wildfire, drought, and a growing population are changing these places now, which makes the gap more urgent to close.

30×30California's commitment to conserve 30% of its lands and coastal waters by 2030
A volunteer in blue gloves collects a soil sample under leafy undergrowth.

How volunteers collected

Two protocols, one standard

Volunteers completed online training and a knowledge check before heading out, with Adventure Scientists staff supporting them throughout. Every sample followed the same protocol, so a vial from the desert can be compared with one from the high Sierra.

Trowel · vial · barcode

Soil samples

Collected at designated sites, scanned into Survey123, and photographed so labs could verify every record.

Sweep net · aspirator · ethanol

Insect samples

Swept from vegetation, preserved in the field, and logged the same way, vial by vial.

What is eDNA?

Every living thing sheds DNA into the soil around it. Environmental DNA lets scientists detect fungi, bacteria, nematodes, and insects from a vial of soil, without collecting live specimens.

In the lab, researchers extract that DNA, copy short barcode regions, and sequence them. The result is a record of what lives in each place that can be compared across elevation, habitat, and wildfire history, and that can point to species science hasn't described yet.

Trusted data from hard-to-reach places, collected by the people who explore them.

“We have hit most of the available ecoregions, and a lot of that's come from the Adventure Scientist samples, so they've really helped fill in those geographic gaps that we desperately needed.”
Austin Baker, Natural History Museum of Los Angeles County
“As I was planning my kayaking adventures, I looked for places that also had the opportunity to collect samples. My friend Vlad (in the picture with me), and I are paddling buddies and this project gave us the opportunity to do what we love and contribute to the project as we took a break for lunch. Anytime I plan a kayaking trip, I now look for ways to be involved with scientific research.”
Darrel R., Volunteer

Field day hosts

Conservation groups that brought their communities into the field

Land trusts, conservancies, and outdoor groups across California hosted volunteer field days on the lands they steward, opening their sites and networks to the project.

Big Sur Land Trust
Feather River Land Trust
Sacramento Valley Conservancy
San Joaquin River Parkway and Conservation Trust
Redlands Conservancy
California Nature Art Museum
Sierra Club Military Outdoors
Thank you for opening your lands and inviting your communities to take part.

What happens next

From samples to decisions

Lab analysis is underway. As results come in, they join the California All Taxa Biodiversity Inventory, a statewide baseline that can help inform conservation priorities, including where California works toward 30×30.

Request the data
  • A soil biodiversity archive at UC Santa Cruz drawn from 112 ecoregions
  • Comparisons of what lives where across elevation, habitat, and wildfire history
  • Leads on fungi and invertebrates not yet in museum collections
  • Geographic gaps filled in the statewide inventory

Thank you.

To the volunteers who carried trowels and sweep nets across California, the partners who opened their lands and labs, and the funders and supporters who made this work possible.

Partners and supporters

Made possible together

Science partners
  • California Institute for BiodiversityProject partner
  • University of California, Santa CruzSoil eDNA analysis and archive
  • Natural History Museum of Los Angeles CountyInsect identification
  • Stillwater SciencesScientific partner and project coordination
Funding
  • Play Forever FoundationProject funder
  • Our donors and supportersWho invest in science-driven conservation
Volunteer gear
  • AllTrails
  • MiiR
  • Osprey

This project was conducted across the ancestral lands of California's Indigenous peoples. We acknowledge their enduring presence and the generations of stewardship over the lands and species documented here.

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